One of the therapeutic approaches for treating type 2 diabetes is to decrease the post-prandial hyperglycemia. Pancreatic alpha amylase inhibitors offer an effective approach to reduce the post-prandial hyperglycemia via cope of starch breakdown. In the present study, ayurvedic seeds, namely Myristica fragrans were subjected to sequential solvent extraction with petroleum ether, acetone and ethanol. These extract were subjected to phytochemical analysis as well as in-vitro alpha amylase inhibitory assay. The results indicated that phytochemical test indicated that ethanol extract of Myristica fragrans seeds containing alkaloid, tannin and flavonoid. In addition, ethanol extract of Myristica fragrans seeds showed appreciable alpha amylase with an IC50 value of 70.39 µg/ml, when compared with standard drug, acarbose (IC50 value 83.17 µg/ml). Thus, the possible mechanism action of the above extracts were due to their inhibitory effects on pancreatic alpha amylase enzyme, thereby reduction the rate of starch hydrolysis leading to decreased the glucose levels. This study suggested that presence of alkaloid, tannin and flavonoid might be responsible for possible inhibitory actions.
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